colorectal cancer cell lines  (ATCC)

Journal: Cancer Biology & Therapy

Article Title: MBD2 suppresses SFRP1 expression and promotes colorectal cancer development by blocking MED19 binding to its methylated promoter

doi: 10.1080/15384047.2026.2667568

Figure Lengend Snippet: Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and HCT116 cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.

Article Snippet: Normal colon mucosa cell line NCM460 (ATCC, CRL-1642 TM ) and the human colorectal cancer cell lines HCT116 (ATCC, CCL-247 TM ) and SW480 (ATCC, CCL-228 TM ) were purchased from iCell, and all had STR identification reports.

Techniques: Inhibition, Expressing, Activation Assay, Binding Assay, Concentration Assay

Journal: Cancer Biology & Therapy

Article Title: MBD2 suppresses SFRP1 expression and promotes colorectal cancer development by blocking MED19 binding to its methylated promoter

doi: 10.1080/15384047.2026.2667568

Figure Lengend Snippet: The effect of KCC07 on recovering SFRP1 expression requires MED19. (A and B) The interfering efficiency of MED19 siRNAs at the protein level in SW480 and HCT116 cells. (C–F) Treatment with KCC07 resulted in increased SFRP1 expression and increased β -catenin expression at both the mRNA and protein levels in CRC cells, while simultaneous knockdown of MED19 abolished these effects. n = 6, ns indicates not significant, *indicates p < 0.05, and **indicates p < 0.01.

Article Snippet: Normal colon mucosa cell line NCM460 (ATCC, CRL-1642 TM ) and the human colorectal cancer cell lines HCT116 (ATCC, CCL-247 TM ) and SW480 (ATCC, CCL-228 TM ) were purchased from iCell, and all had STR identification reports.

Techniques: Expressing, Knockdown

Journal: bioRxiv

Article Title: Enantiomer-Dependent Biological Activity of Cysteine-Coated Ceria Nanoparticles in Colorectal Cancer Cells

doi: 10.64898/2026.04.27.721174

Figure Lengend Snippet: The selected models represent a clinical spectrum ranging from healthy colon tissue to aggressive metastasis. CCD-18Co serves as the non-malignant, healthy fibroblast control. COLO-201 is a highly proliferative adenocarcinoma characterized by high stress sensitivity and low functional A20 expression. DLD-1 is an MSI-H model with intact, albeit modified, A20 feedback (SNV:single-nucleotide variation). LoVo represents a metastatic MSI-H line harboring a Loss-of-Function (LOF) A20 mutation, maintaining resilience through a robust GSH-mediated antioxidant shield. (Gradient bars: Green = Low; Red = High). Created with BioRender.com .

Article Snippet: The colorectal cancer cell lines DLD-1 and COLO-201 (ATCC) were maintained in RPMI-1640 (Sigma-Aldrich, St. Louis, MO, USA, R8758), while LoVo (ATCC) was cultured in F-12K Medium (HyClone, Cytiva, USA, Cat# SH30526.0).

Techniques: Control, Functional Assay, Expressing, Modification, Mutagenesis

Journal: bioRxiv

Article Title: Enantiomer-Dependent Biological Activity of Cysteine-Coated Ceria Nanoparticles in Colorectal Cancer Cells

doi: 10.64898/2026.04.27.721174

Figure Lengend Snippet: (A & B ): Cell viability of COLO-201, DLD-1, LoVo, and CCD-18Co cells treated with D-Cys@CeNP (A) and L-Cys@CeNP (B) for 24 h at increasing concentrations. (C & D) Cell viability of COLO-201, DLD-1, LoVo, and CCD-18Co cells treated with D-Cys@CeNP (C) and L- 34 C 0 ys@CeNP (D) for 72 h at increasing concentrations. (E) IC 50 values for D-Cys@CeNP and L-Cys@CeNP treatments at 24 h and 72 h for all cell lines. (F) Summary table displaying the mean IC 50 values (µg/mL) for each treatment condition across all cell lines. The results represent the mean ± SD of three independent biological replicates (n=3). The dotted vertical lines indicate the IC 50 values, which are also displayed in the figure. Statistical significance was analyzed using one-way ANOVA followed by Tukey’s post-hoc test: (ns = non-significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: The colorectal cancer cell lines DLD-1 and COLO-201 (ATCC) were maintained in RPMI-1640 (Sigma-Aldrich, St. Louis, MO, USA, R8758), while LoVo (ATCC) was cultured in F-12K Medium (HyClone, Cytiva, USA, Cat# SH30526.0).

Techniques:

Journal: bioRxiv

Article Title: Enantiomer-Dependent Biological Activity of Cysteine-Coated Ceria Nanoparticles in Colorectal Cancer Cells

doi: 10.64898/2026.04.27.721174

Figure Lengend Snippet: h. The Selectivity Index is defined as the ratio of IC50 values in healthy control cells (CCD-18Co) to those in CRC cell lines (LoVo, DLD-1, and COLO-201). (A) Enantiomeric Comparison at 24 h: Comparison of SI values between D-Cys@CeNP (orange) and L-Cys@CeNP (brown) after 24 hours of exposure . (B) Enantiomeric Comparison at 72 h: Comparison of SI values between D-Cys@CeNP and L-Cys@CeNP after 72 hours of exposure. (C) Time-dependent analysis of D-Cys@CeNP selectivity across CRC cell lines. Over time NPs become more selective in COLO-201 . (D) Time-dependent analysis of L-Cys@CeNP. The horizontal dashed line at SI=2.0 represents the baseline for selectivity. Data are presented as mean ± SD (n=3). Statistical significance is indicated by asterisks (, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Article Snippet: The colorectal cancer cell lines DLD-1 and COLO-201 (ATCC) were maintained in RPMI-1640 (Sigma-Aldrich, St. Louis, MO, USA, R8758), while LoVo (ATCC) was cultured in F-12K Medium (HyClone, Cytiva, USA, Cat# SH30526.0).

Techniques: Control, Comparison

Journal: bioRxiv

Article Title: Enantiomer-Dependent Biological Activity of Cysteine-Coated Ceria Nanoparticles in Colorectal Cancer Cells

doi: 10.64898/2026.04.27.721174

Figure Lengend Snippet: (A) COLO-201 cells treated with D-Cys@CeNP and L-Cys@CeNP for 24 h; ROS fold change comparison. (B) DLD-1 cells treated with D-Cys@CeNP and L-Cys@CeNP for 24 h; ROS fold change comparison. (C) LoVo cells treated with D-Cys@CeNP and L-Cys@CeNP for 24 h; ROS fold change comparison. (D) CCD-18Co cells treated with D-Cys@CeNP and L-Cys@CeNP for 24 h; ROS fold change comparison. (E) Mechanistic Efficiency Index values for D-Cys@CeNP (orange) and L-Cys@CeNP (blue) across four cell lines: COLO-201, DLD-1, LoVo, and CCD-18Co . (F) ROS fold change at IC₅₀ concentrations in colorectal cancer and control cell lines treated with D-Cys@CeNP (red) and L-Cys@CeNP (blue) at their respective IC₅₀ concentrations.The vertical dashed lines indicate the IC 50 values on the X-axis. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: The colorectal cancer cell lines DLD-1 and COLO-201 (ATCC) were maintained in RPMI-1640 (Sigma-Aldrich, St. Louis, MO, USA, R8758), while LoVo (ATCC) was cultured in F-12K Medium (HyClone, Cytiva, USA, Cat# SH30526.0).

Techniques: Comparison, Control

Journal: bioRxiv

Article Title: Enantiomer-Dependent Biological Activity of Cysteine-Coated Ceria Nanoparticles in Colorectal Cancer Cells

doi: 10.64898/2026.04.27.721174

Figure Lengend Snippet: (A) Expression levels of TNFAIP3, IKBKG, and NFKBIA in all cell lines (COLO-201, DLD-1, LoVo, and CCD-18Co) after CeNP treatment. (B) Comparison of TNFAIP3, IKBKG, and NFKBIA expression within each cell lineand each nanoparticle treatment (D-Cys@CeNP vs. L-Cys@CeNP) across all cell lines. (ns = non-significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: The colorectal cancer cell lines DLD-1 and COLO-201 (ATCC) were maintained in RPMI-1640 (Sigma-Aldrich, St. Louis, MO, USA, R8758), while LoVo (ATCC) was cultured in F-12K Medium (HyClone, Cytiva, USA, Cat# SH30526.0).

Techniques: Expressing, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Transcriptomic Profiling Reveals Isoform-Specific Regulatory Roles of miR-196A and miR-196B in Colorectal Cancer Cells

doi: 10.3390/ijms27093959

Figure Lengend Snippet: Generation and functional validation of miR-196 knockout SW48 cells. ( A ) Sequence alignment of miR-196A and miR-196B showing a high degree of similarity between the two miRNA isoforms. ( B ) Quantitative RT-PCR analysis of miR-196 expression in parental SW48 cells and in miR-196A-KO or miR-196B-KO cells. Relative expression levels were normalized to an internal control RNA and presented as fold change compared with control cells. ( C ) Cell proliferation assay showing the relative growth rates of control, miR-196A-KO, and miR-196B-KO SW48 cells. Data represent the mean ± SD from independent experiments. Statistical significance is indicated (* p < 0.05, *** p < 0.001).

Article Snippet: The human colorectal cancer cell line SW48 (ATCC Cat. No. CCL-231) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Functional Assay, Biomarker Discovery, Knock-Out, Sequencing, Quantitative RT-PCR, Expressing, Control, Proliferation Assay

Journal: International Journal of Molecular Sciences

Article Title: Transcriptomic Profiling Reveals Isoform-Specific Regulatory Roles of miR-196A and miR-196B in Colorectal Cancer Cells

doi: 10.3390/ijms27093959

Figure Lengend Snippet: Identification of differentially expressed genes following deletion of miR-196 isoforms in SW48 cells. ( A ) Volcano plot showing the distribution of differentially expressed genes (DEGs) in miR-196 knockout cells compared with control SW48 cells. Significantly up-regulated and down-regulated genes are highlighted based on predefined statistical thresholds. ( B ) Summary of DEGs identified in the transcriptomic analysis, showing the numbers of significantly up-regulated and down-regulated genes in miR-196 knockout cells relative to control cells. The vertical dashed lines indicate the fold-change thresholds, and the horizontal dashed line represents the statistical significance threshold (adjusted p -value cutoff). The grey shaded area indicates genes that are not significantly differentially expressed. ( C ) Venn diagram illustrating the overlap of DEGs between miR-196A-KO and miR-196B-KO cells. A subset of genes was commonly regulated by both miR-196 isoforms, while additional genes were uniquely altered in each knockout condition.

Article Snippet: The human colorectal cancer cell line SW48 (ATCC Cat. No. CCL-231) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Knock-Out, Control

Journal: International Journal of Molecular Sciences

Article Title: Transcriptomic Profiling Reveals Isoform-Specific Regulatory Roles of miR-196A and miR-196B in Colorectal Cancer Cells

doi: 10.3390/ijms27093959

Figure Lengend Snippet: Functional enrichment analysis of differentially expressed genes following miR-196A or miR-196B deletion. ( A ) Functional categorization of differentially expressed genes (DEGs) identified in miR-196A-KO cells compared with control SW48 cells. The pie chart shows the percentage distribution of genes associated with major biological processes. The percentages may exceed 100% because individual genes can be associated with multiple functional categories and are therefore counted in more than one category. ( B ) Distribution of significantly up-regulated and down-regulated genes in each functional category in miR-196A-KO cells. ( C ) Expression profiles of representative genes altered in miR-196A-KO cells. Each point represents normalized RNA-seq expression values from independent samples. ( D ) Functional classification of DEGs identified in miR-196B-KO cells relative to control cells. The percentages may exceed 100% because individual genes can be associated with multiple functional categories and are therefore counted in more than one category. ( E ) Numbers of significantly up-regulated and down-regulated genes in each functional category in miR-196B-KO cells. ( F ) Expression patterns of representative genes affected by miR-196B deletion.

Article Snippet: The human colorectal cancer cell line SW48 (ATCC Cat. No. CCL-231) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Functional Assay, Control, Expressing, RNA Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Transcriptomic Profiling Reveals Isoform-Specific Regulatory Roles of miR-196A and miR-196B in Colorectal Cancer Cells

doi: 10.3390/ijms27093959

Figure Lengend Snippet: Hierarchical clustering and reproducibility assessment of RNA-seq data from miR-196 knockout SW48 cells. ( A – C ) Heatmap visualization of differentially expressed genes between miR-196 knockout cells and parental SW48 cells using fold-change thresholds of >1.5 ( A ), >2.0 ( B ), and >3.0 ( C ). Hierarchical clustering reveals distinct expression patterns associated with miR-196 deletion. ( D ) Pearson correlation heatmap showing strong correlations among biological replicates and experimental groups. Color intensity represents the Pearson correlation coefficient (r), ranging from −1 to 1, where values closer to 1 indicate stronger similarity between samples. The numerical values in each cell correspond to the correlation coefficients. ( E ) Pairwise scatter plot matrix demonstrating high concordance in gene expression profiles across samples. The red line represents the linear regression fit. Asterisks (***) indicate statistical significance ( p < 0.001).

Article Snippet: The human colorectal cancer cell line SW48 (ATCC Cat. No. CCL-231) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: RNA Sequencing, Knock-Out, Expressing, Gene Expression

Journal: International Journal of Molecular Sciences

Article Title: Transcriptomic Profiling Reveals Isoform-Specific Regulatory Roles of miR-196A and miR-196B in Colorectal Cancer Cells

doi: 10.3390/ijms27093959

Figure Lengend Snippet: Functional enrichment analysis of genes altered following miR-196 deletion. Gene Ontology (GO) and KEGG pathway enrichment analyses were performed using differentially expressed genes identified from RNA-seq analysis of miR-196A-KO and miR-196B-KO SW48 cells. ( A ) Bubble plot showing significantly enriched GO terms and KEGG pathways in miR-196A-KO cells. Functional categories are grouped into biological process (BP), cellular component (CC), molecular function (MF), and KEGG pathways. Bubble size represents the number of genes associated with each term, and color intensity indicates the −log10( p -value). ( B ) Bubble plot showing enriched GO terms and KEGG pathways in miR-196B-KO cells using the same criteria. ( C ) Distribution of up-regulated and down-regulated genes contributing to each enriched functional category in miR-196A-KO cells. ( D ) Distribution of up-regulated and down-regulated genes contributing to each enriched functional category in miR-196B-KO cells.

Article Snippet: The human colorectal cancer cell line SW48 (ATCC Cat. No. CCL-231) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Functional Assay, RNA Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Transcriptomic Profiling Reveals Isoform-Specific Regulatory Roles of miR-196A and miR-196B in Colorectal Cancer Cells

doi: 10.3390/ijms27093959

Figure Lengend Snippet: Differential gene expression patterns in miR-196A-KO and miR-196B-KO SW48 cells. Radar charts display the top ten most highly upregulated and downregulated genes in each knockout condition. Left panels show average normalized expression values (log2); right panels show fold change relative to parental SW48 cells (SW48-vector). ( A ) Top ten upregulated genes in miR-196A-KO cells, including RIMS2 , ADGRL2 , and LAMA2 . ( B ) Top ten upregulated genes in miR-196B-KO cells, including PCCA , LAMA2 , and AKAP12 . ( C ) Top ten downregulated genes in miR-196A-KO cells, including KRT14 , KLK11 , and KRT16 . ( D ) Top ten downregulated genes in miR-196B-KO cells, including KRT16 , KRT14 , and KRT19 . ( E ) qPCR validation of selected miR-196-associated genes ( NT5E , PRRX1 , KITLG , CLDN4 , and FLG ) in parental SW48 and miR-196 isoform knockout cells, showing isoform-dependent differences in gene expression. Data are presented as mean ± SD ( n = 3).

Article Snippet: The human colorectal cancer cell line SW48 (ATCC Cat. No. CCL-231) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Gene Expression, Knock-Out, Expressing, Plasmid Preparation, Biomarker Discovery

Journal: International Journal of Molecular Sciences

Article Title: Network Pharmacology and Molecular Docking-Based Approach Revealing the Potential Anticancer Compounds and Molecular Mechanisms of Paris polyphylla Against Colorectal Cancer

doi: 10.3390/ijms27093874

Figure Lengend Snippet: Cytotoxic effects of Paris polyphylla rhizome extract (PPRE) on colorectal cancer cells. SW480 ( A ) and HCT116 ( B ) cells were treated with increasing concentrations of PPRE (0–80 µg/mL) for 24 and 48 h. Data are presented as mean ± SD from three independent experiments. Statistical significance compared with the untreated control group was determined using one-way ANOVA followed by Tukey’s multiple comparison test (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Human colorectal cancer cell lines SW480 and HCT116 (ATCC, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Control, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Network Pharmacology and Molecular Docking-Based Approach Revealing the Potential Anticancer Compounds and Molecular Mechanisms of Paris polyphylla Against Colorectal Cancer

doi: 10.3390/ijms27093874

Figure Lengend Snippet: qRT-PCR Validation of STAT3, EGFR, SRC, IL-6, and AKT1 mRNA expression in SW480 ( A – E ) and HCT116 ( F – J ) cells following treatment with PPRE (0–10 µg/mL). Gene expression levels were normalized to the internal control and expressed as fold change relative to untreated control (0 µg/mL). Data are presented as mean ± SD of three independent experiments. Statistical significance versus the untreated control was determined using one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Human colorectal cancer cell lines SW480 and HCT116 (ATCC, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Gene Expression, Control